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CRIMIC CR71383

Gene Desat2

CG5925

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	III:12436248..12437857	87B10-87B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCCACCACAAATACTCGGAAACTG	GATTACCTACTTGTCGTATGGGCG

Images

Construct Design Sequences

sgRNA sequence	ACAAGAGGGGGGACCCCTGGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AACGCCACCAGGGGATTTTTCTTCTCGCATGTGGGCTGGCTGCTCTGCAAAAAGCATCCGGATATTAAGGAAAAGGGAAGGGGACTGGATCTCTCCGACCTGCGCGCCGATCCCATCTTGATGTTCCAACGAAAGTAATTATTTAATAGCTTTTCATGGATCAAAAGAATCTAAATGCAAATTACAAGAGGGGGGACCCC	TGGAGGGTTCAAGTAGAGGACTCATTTAATAAGTTATAAAAATAATTTATAGGCACTATTATATCCTCATGCCGCTGGCCTGCTTCGTTCTGCCCACCGTTATTCCAATGGTTTACTGGAATGAGACGCTGGCCTCCTCTTGGTTTGTGGCCACCATGTTCAGGTGGTGCTTCCAGCTAAACATGACCTGGCTGGTGAAC

 

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