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CRIMIC CR71384

Gene Mco4

CG32557

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:17852958..17856129	16E1-16E2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTTGTTTTGGATATTAAGCAAATCGG	AAGGATGCCGCTGGTTGTCCTT

Images

Construct Design Sequences

sgRNA sequence	CTATATATCAACTTGAGTGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTGCCATTTTATGCGAGCCACTTGGAAAATGAATAATATTGAACTATATTGGCATTTAAGCACATAAAACCAATCATGAAATACCAAGGAGTTGACACTAAAAATAAAAATATATTTTTGAAATAACGAACAAACATATTTTAAGGACAACTGGTCTTTTAACTAAAAACAAATGTACTAAATCTATATATCAACTTGAG	TGTTGGATCTAAAATTATTTATAATTATTCATGATACTCTGAATGCCAAATAAAAATATTTAAAACGGCAATAACTAATTAACTAAGCTTATGCTAGTGAATATGAAATTTGCATTAAATCCCAGTTTCCAAACAACACCAACAGATGCCAGTGGCAAGCGGATCGTTAGCAAGTACGAGCGCATCATGCAGATGTATCC

 

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