CR71386

CRIMIC CR71386

Gene anne

CG32000

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	4:132220..143123	102A3-102A		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGAGTTGCTGGACCTACACATG	GTAAGCACCCTCTGAAACAGAGA

Images

Construct Design Sequences

sgRNA sequence	GCTATTAATGTGCCACTGAACGG
sgRNA2 sequence	GTAAATTGTACTGTAACCTAAGG

left_200bp	right_200bp
ATTGAATTATTATTAGTAAAGATATTACGATTGGGAAATTAGCTTACAGTTGAATATTAAAAAACGTTATTAGATTCATTACATATTACAATTTGCATTCCATTTCAGAAATTTATCACTTGGTCAAACAACAAGGAAGAGCCTATTAAAGAAATTGAACTAATACCTGATTTTGCTTGGGTAGCTATTAATGTGCCACT	CTAAGGAATTACTTTTAGGAATTTCCATTATTAATACAGTAGAAAATGTAGGCAAAATTATTTAGTTCCTATTTAGTTTTTTTAGTAATCTTAGTATTTCTAATTGTACTTACATCAAAGATAACTTTGTATTAGCTATGAACACTTTTGTCTTTCTATGTTTAAAACGGCGTTGTTTCTTGATTATAATTAAGTGTTTG