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CRIMIC CR71387

Gene Cirl

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:8614722..8625203	44D4-44D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTGTAACCAGGTCATTAGATTGACTC	TGGCATGTGGTGACATGTATTACAATAC

Images

Construct Design Sequences

sgRNA sequence	TCTTATCGGCAATGCTCATTCGG
sgRNA2 sequence	TTCGACTAACGACTAATACTAGG

 

left_200bp	right_200bp
GATATTGCACAAGTAAATTAAATGGAACCAATATCAATAAAAGAAAAGGATAGTTCTCCTTTTCCTTTCTAACGAAATACAACATGTCTTAAGTTATTAGTCATGTGCCTTAAAATAATAATAATAATAATACCAAACATTATAAATGTTATCTTCCCTTTCAGTTTGAATACTTCAAACCGAGTGAAACACTGCCGAAT	ATTAGTCGTTAGTCGAAATATGGAGCATTGTGTGAAACAACATGCTGTCATGCTATACGTGCAGTGATTCGAAAATCCCAAAACTTTATAAATGTATATGAAATAACTAAAACCTAGACCATAGGGCACATCCATACCCCTGCGGTGCCGTGCAAGCTTAACCAAAACTCGAGCGATGGGGGGTTGGAATTGGAATTGGA

 

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