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CRIMIC CR71388

Gene Irk1

CG44159

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	III:23168317..23185642	94E3-94E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGTACATCTTGTTGTTCCGTTTCAG	CAACCAGGGTGGTGAAGATGTC

Images

Construct Design Sequences

sgRNA sequence	GGCGTTATGCAAACCCGCGAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGCGTGTTCGAAAACGTGTGGTCTTCAAGCACGGCGAGTGCAACGTTGTGCAGGGAAATGTGGCCAAGAGACGGCGTCGCTATCTGCAGGTGAGCGGTGGGCGGTGGGCGGTGGGAGTTACGGGGCGTGGCAGTGCGTGGCAATATCTAGTTGCCGGCCCACTTGCGTTCGATTTGTGGGGTATATTTAGACTTCCTTCG	CGGGTTTGCATAACGCCTGTCGCCGCGAGACTTGGGACGGCAATTATGTAAATTGATTTGTGCTCCAAAATTAAATGTATGTCGAAAGTATTCAAAGAATTTATGGGACTTAACCATCTAGTAGAATAGACTATATATGGATACACTATAATTGCCATAGGTATTGATTGATATGTTTCTGAAAGCAAATATTGTTTTCA

 

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