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CRIMIC CR71392

Gene Naglu

CG13397

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:8408888..8411868	29C3-29C3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCCTCGCATTTCAGAACTCTAC	GATGTATGATGCAAAGCCACATTTAAG

Images

Construct Design Sequences

sgRNA sequence	CACACACGGCAGTAAATAGACGG
sgRNA2 sequence	TGGCAGTTGTATTGAACTATAGG

 

left_200bp	right_200bp
TCATTGTTCAATCTGGCGATAAGAAGAAAGTGATCGGAATCCAGCTGCTCTTCTCTTCCATTGTGTTCTCATCGGGTTCACTTAGCGCTCTTTCCTCCGTTCCCGCATTCTTCGGGAGAGCCCCAATTGCGAGAGAAGGAGAAGCTGTCAGTCAGTCAGTCACGTGGGCCCCGTTGACATTGACACACACGGCAGTAAAT	GTTCAATACAACTGCCACAACAAACGTTTAATAAACTTTTCTCTAAAATTGACTTTAAATATTCGAAATAAAAATCTTTTGACACTAGTTGCTCAAGGATTAAACGAAAACATTTCAGATATACGGATATCCCACCAAACTTTCAAAATTGATTGGCATCAATCCAATAATGTTTCAATCAATTGCGAAATTCCACGAGA

 

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