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CRIMIC CR71393

Gene Hgsnat

CG6903

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:4918730..4921648	4D6-4D6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGACAAGAACGCAAATCACC	GTGTGACCACTGTACCTTGTGACT

Images

Construct Design Sequences

sgRNA sequence	ATTAAAATCGAATTCACTGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTGGAATATACGGAGCCGAAGTGGCGCCACCTGGATATGCGCGCGCTATCCGTTGACGAGTTTTACCTGTACCTGCGCTCGGAGTTCAGCAGCCAGACCCATCTGTACTCACTGTCCGACGAATGCTACACGGTAATAAATTGATGGCCCCATGTAATCACATCGCGCAATCGGTTTCCCTTTCAATTGGGTGGCCATCA	GTGAATTCGATTTTAATTGTATTCTTTGCCGTTTTCAATTCGCAGTGTCCGTATACCAAAGTGAAAGCTCTGCCCGCTGCCGACGTCGCTGCCGTCGTCGTTGAACCGTTACGTTTGAGTTCGGCATATTCGCTGAATTTCAAAATATTCGACCTCGATCAGGGTCACTATGCCTACGACAATGTGTGAGTATCACTCAA

 

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