CR71394

CRIMIC CR71394

Gene CG15117

CG15117

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:19122231..19129300	56B5-56B5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	ATGCCAAGGAGTTGAGCAAGAGCA	TGGGTGCTGCCAGCACAAATGTAT

Images

Construct Design Sequences

sgRNA sequence	TGAGAATTCCTTTATTTCAGTGG
sgRNA2 sequence	CTCGGTAAACTTATTTACAGTGG

left_200bp	right_200bp
CGCAAGTTCTTTGTGCCCCGATCGTGGTCAAAGGATCAGAGGATATGGCTGCGATTCGGCAGTGTTCACTATGAGGCATATGTGGTGGGTACTTCAAATATATTCTCATGCAATCAAGTACAAGTATTTAGTTATTTAGTTGTGATAATCAATATTTTCTGGTAGAGGATCATATAAAATTAATGAGAATTCCTTTATTT	TAAATAAGTTTACCGAGAAAAACTTTATCCTCGGACTTGCATTTTAGCCCGACTACCATTCCGCTGCATTGCCCTGTACAATATAAAATGGTTAGCTCTAGTTGATTGTATTTTTGTATGTATTTGTATGTTTAAGAGAATAAATGAAAGTGCATTATTTATATTTACCACACAAAATTTGGAAAATTTTCAAATAGCTC