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CRIMIC CR71396

Gene Taf4

CG5444

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	III:16113212..16121651	72D7-72D8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCACCCAAACACAAACCCTAG	GGGAAACTTTTCGGCATTGGGAT

Images

Construct Design Sequences

sgRNA sequence	ACTACTATCCGATTCGAGCGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTAAATCCAACCACCATGCTGTCACCCTGCCGGCGGGTCTAGTCTCAAGTGCACCAGCAGGAATCGTAACCATGACCAAGACCATCAATCAGGTATCCATATTAAAGCTAAACCCACTCTTCGGGCGCTAGGCGATCGCAACTCGGGCGTATCACTTCGTCCAAATCTGATCCCCAATCAAAACCCCCTCAATTCCTCGC	TCGAATCGGATAGTAGTGGCCGCACACACTTTGGTTGTATACTTTTATTATGCTTTCATCTGTCTACCTCGCTGCCCGAAATTCCCATATCCTTATCCAAATGGGGTTAGTCCCAGGCTTGATAACGTATTTTACGCTTAAAAAATATCCTTAATTCATTAATGTGAAATCTGAAACTTATATCTGAGTTGAAATCTGAA

 

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