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CRIMIC CR71399

Gene Nach

CG8178

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	II:16653205..16655046	53C14-53C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTGTGCACCTGGTGTGGCTCTTA	ATGTCCATTGGCTGACCTATGGCA

Images

Construct Design Sequences

sgRNA sequence	GACTGGAGGAATAAATCAAGTGG
sgRNA2 sequence	AGAGTATATCAAAGTTCATTCGG

 

left_200bp	right_200bp
GGCGCGCACCTTCTACATGGACTATCGCAGCAGTCCGACGCGTATGAATGTGGAAAGTGATAACACACCGGTTAATAGACTCTATTTCCCACCTGTCACAATTTGTCCAGATGTACTCTTCAACATGCAAAAGTCCGAGGCCTTTCTGAACACTCTGTAAGTGTATGTTATTTAAAGGTCTGTACAAGGATGTGCCACTT	GAACTTTGATATACTCTTTTGGAAGCGGTTGTTGTTTTCTTTAGGAAGAAATACATATACTTTTATTGTGTTGTCTTGAAGAACTAACATTTAAACTTAGATTCGAATTCGACTATGTGATGTGATTGTAACATATGCGTTAAATCCTATGCAAGTTACAATGTTTATGCGGTTATCTTTTACGGTACTAAACCAAGCAG

 

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