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CRIMIC CR71400

Gene Lam

CG6944

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	II:5542480..5546642	25E6-25E6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGAGATCGATATCAAGCGTCTC	AGTGTCTCCTGTTCCAGGTTCTTC

Images

Construct Design Sequences

sgRNA sequence	CAGAAAGTGAGGAATATCATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCTGGACAAGAAGACCAAGGAGTGCACCACTGCTGAGGGTAAGTGCGTGGAAACTGAATCGATAAGAAAACCTTATAATTGTAGGGCAAAGAAAGTAGAGTAATGATAAAATTAAAAGTAAGAAGAGACAAAAGGCGTGGTTTCAAGCAAGAACGAAACATTCATTTAGTTGCTGAACTGTATATAATGATTATCCCATG	ATATTCCTCACTTTCTGTGACAAAATCATCCCTATTCTGGCATTTAACGAACATTAATTGATTGATTATCCCAACCCGCAGGCAATGTCCGCATGTACGAGTCGCGCGCCAACGAGCTGAACAACAAATACAACCAGGCCAACGCCGATCGGAAGAAGCTTAACGAAGACCTGAATGAGGCGCTAAAGGAGCTGGAGAGA

 

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