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CRIMIC CR71406

Gene Dic4

CG18363

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:18696402..18698412	75D6-75D6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGCCTGGCTAAGCATTTCACAG	GCGACCAGTTGTGGTTGATGTTTC

Images

Construct Design Sequences

sgRNA sequence	GAATCCGAATGCACGGTGAAAGG
sgRNA2 sequence	AATTGTACTTCTCCCCTCCCAGG

 

left_200bp	right_200bp
AAATTTGTCCCAAGTAAGAAGCCGTTTTATTTAAAAAAGCTCAATCTGTATTGATAGTTTACAACGATAGCAACAGTATTATAATGTTAAAATTGTCAGTGAAAGCAAAAAAGAAACACATAAGCCTTTCCCTCCCAATCAAAAGCCAATTTCTTTAAATTAGGGCCAATCTAGAATCGTCCAGAATCCGAATGCACGGT	AGGGGAGAAGTACAATTGAGACGATCGTGCTGCGCATTCGGAATTTGACAAAAATACCATGTGAACTTTTACCAACGTTTTTTTACGATAAAGATTTCCATGTTAATTCAAAGAAGAATTTAATTTCGCTTACTAATGGAATAAGAAGTCCTCGTAAAGCAGAATTATAGCAAATAAAAAATCGAAGCCCAGTGCTCACT

 

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