CR71408

CRIMIC CR71408

Gene CG18418

CG18418

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:4688929..4690070	64B13-64B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGCCAAAGGTGTTGGCCTGTTA	GCTGAATGAATGGAAATCCATGTTAGG

Images

Construct Design Sequences

sgRNA sequence	GAAGATATAATAAGTATGTTTGG
sgRNA2 sequence	ATTTCCATTTGTTTGGGATGAGG

left_200bp	right_200bp
AACATTTCCATGGACTGGATCTCTCCTTTGGTTGAAAGAACTATGGTCAAGTGGAGGACCTCTAAGAATTATCTTTTGGGAAAAAGTCTTGCTTGTTGTGGAAGGCTTTGTTGTGGATGTCAACTCCATAAAAACCCCCACATACGAATTTGGCTCGTTTCCAACCATACACTCTCAATTCACATCTCTTTTTCCCAAAC	CCCAAACAAATGGAAATTGATACGATGTCTGTGTAAAGCGCGCTTGTTCTGTAAAGTAATTCATATCTTATATACAACAAAAAAATAGTTTATTATGCAAGACTGAATTTAATTAATCCAAATCATGGGAATGGTGTGGTTTGATATATAAGAGAATCGACTGATTTCCTAAGAATTTTTATTACGAAAATATGGTATCT