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CRIMIC CR71411

Gene Ucp4C

CG9064

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:5968566..5969761	26A4-26A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTACTTCAATCAAAGGACCATACTTGAG	TCTTGTTGGTCACCAAGTACTCC

Images

Construct Design Sequences

sgRNA sequence	TCCATTGTAAACTCGTAATTTGG
sgRNA2 sequence	TTTATAATATGTATAATAGATGG

 

left_200bp	right_200bp
ATCCTGGGATAGCATTCCTTGTATGTATATAGATGGATATTATATATACTAAAGAGAAACTAGTCCAATCGCAGACACCACCTGTTGGTATGTCGTCTATTTATAGTAACACTTAAATTGTAGCTACCTAAACACATTTCAAACAATTCTAAACAAACAATTCCAAACATACAATTCCACTTACCACTTACCGACCAAAT	ATTATACATATTATAAATATAACTTTAATCCCAATCCTATTCGTTAATATGAAATGATTTTGTTGTCTTTAATTTTGCTTTATTTTATTTTTTATATTCCAATGCACACAACAAACACTCAAGATATTTCTCTTAAATGGGAAAAGGCGTTAACACAGTCTTTAGGCCAGCAGAATGGGACAATTCGGAGGAGAAGGAAA

 

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