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CRIMIC CR71414

Gene Lk6

CG17342

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:11750931..11764457	86E17-86E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGACCTTGTATGCATATAATAACTATGG	GCTCAACATCTAAGAGCTGTGACG

Images

Construct Design Sequences

sgRNA sequence	GCATTCTAGACCATTCGCAAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTCATTTGCTGCGCAATAATTTTCTGCTTCTTTACAGCACACAAACACAGGCGTGCAGATAACGTTCGTGTGTGATGTGTGCAAGAGTGTTAGTGTCTCTGCCTGCCCGTGAATTCATGGTGTCAGGAGAAGAAGGTGGTTTAAGAAAAAAAAGAGTGAGTTGTTGGGGTGGCATCTCTTCAGCATTCTAGACCATTCG	CAAGGGAACGTGTAAATAACATGTATATGAAATGTTTAATTAATTTGCGGAGTTACAAAAGAATTCTTTTAACTGATTAAAGTGTGTTTTTAGATGTAGGCTGAATAAAATGTTAACGAAATCTAAATTTTTGAAATTCCAATTAAAATATTTGCATTTTTAATTGAACAATATTAATATACCTACATGTATGTATGTAT

 

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