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CRIMIC CR71415

Gene Obsc

CG33519

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:23995543..24014254	60B1-60B2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTGGGATCTGGGATCTGTCGTAA	ATTCTGCCACAGAGTAGGAGGCA

Images

Construct Design Sequences

sgRNA sequence	GAGAATTGGTTGTCAACGAGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTCATCTACACATAAATCCGTTGAGTTAATCGTAAATAACTTTGTTTAAACTATTCTTCATGTGTTACTCTTCATTGTTTACACACGTTGTTTTCTTTACAAATAACACTTTATGTGTGTCTACCTGTTTTCCCACATTGAATCCCAAAACCAATCCCATCCTTGCAAGTTCCTCTTTAGAATGAGAATTGGTTGTCAAC	GAGGGGTTCTTGGAATCTCTGGATTCTCGGGATTTCAGGAATGCCTCTGTCTAAAATTGAATATTCCGTTTTGGCCCACTACTAATAAACTTTTCCGACTCTGGCCATCCGTAAAGCCAAAACTCGGGACTACGACCGGGGAACCTCCTATGACAGCACCGTTGAGCGATCGCAGTATGGCATAAGCTCCAGGCGGGATC

 

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