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CRIMIC CR71430

Gene Ac3

CG1506

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:21632587..21647964	39E3-39E3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAACGACAGCGATGTGGC	TCAAGAGAGTACGTGAATGTTGGC

Images

Construct Design Sequences

sgRNA sequence	GCGTTAAAGACCTTGTCGGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCAGCACGTGCTTAACGAACTCTACCACAACTACTCCATCAAACAACGCCGCAGTGGATTGAAGTGGTTTGTCTTTGCGGCCGCGTTGTTTAACATATGGACCATCGGAATTCCCTGGGATCAGTCGGCCCCTACGAGAGGTGAGCTATGAATAGAAATAAATTGTTTAAAGAAATTAGTATCGCGTTAAAGACCTTGTC	GGATGGATGTGTACATAACTTATACCTCATATCTGACAATGTATATTTATAAGAATAACTTTTTAAATGATTACAGTCGTGTAATTTCCTGTTGGAATCAATTTATCAATTTATTTTATTCATTTTTATATATGTCCAAAAATAAATTTTCTTCAGCTTCAGTTATAAGCTTGGATGGACAGAGTACTTATTTAGGTATA

 

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