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CRIMIC CR71431

Gene CG43373

CG43373

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:16491838..16543370	73A3-73A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGTGTTGGTTGGAAGCTATG	ACGTTTTGCCCACTCATGCACACT

Images

Construct Design Sequences

sgRNA sequence	GCTAAGCTGACATGCACAGGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATGACTTACAATGTTTATTTTCCAATAAAAGCAAACTGAAAGTATCTTTTCTATAACTTTTAGTGCTATATATGGTTTTAGATTCGTTACTTTGCACAAACGAGTTTTAATGCCAACTAACAAGCCACGTCTGGGAACTTACCCACACACTTGCAGGCAGACACCCACACACACACTTGCATGGCTAAGCTGACATGCAC	AGGGGGCCACTTAACAGCTGCACACGTCTGCGCGTATGTGTGTGTGTGTGTGAGTATTTTTATTTGCGTTCGTTGTCAAGAGTCAATTGACAAAGTTTAGCAAATTGAAAAGGAAAAAAAAGAGAGAGGAGCTACAAAAAAGCATTCTGGCATTCAAATGGGCGGCAATTTGCTTAAAAACGCCGCGGGGATCCAAACAC

 

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