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CRIMIC CR71432

Gene hts

CG43443

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:19397032..19424903	56D3-56D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCTGAGCGTCAAGTTGAAGAAG	AGTTGCTGCTCTTGAAGACG

Images

Construct Design Sequences

sgRNA sequence	TGGTGGTGGTGAGTGTTTGGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTGAATTCGGGAAAAACCCTTTTTCTCCATTTAATTGCAGCATTTTAACTGAAATCATGACTGAAGTTGAGCAACCGCCACAGAATGGCATAGATCCCACTGCCGGCGAAGATGATGACAATAGCAAAGCACGTCCCGCGGATATTGAACAGGTAAGTGCAGGCCGTCGAAGTCCCAACTGGATAACAACCCCTCCACCA	AACACTCACCACCACCACTTTACTTTCTCCTCCTCCAGGATATGCGCGAAATGGAGCGTCGCAAGCGAGTCGAGGCTATAATGGGCTCGAAACTGTTCCGCGAGGAGCTGGAGCGTATCGTGGACTCGGCGCGCGATGGAGGTGCTGGCGCTAGTGGCATCCTGCAGCAGCTGTCGGACATTGTGGGCGTACCAGTGTCC

 

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