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CRIMIC CR71433

Gene Rpn13R

CG6789

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:5131898..5132985	4F1-4F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACCAAACGGCTCACGCTGAAGTA	GTTTTCATCGCTGCCTTGCTTCT

Images

Construct Design Sequences

sgRNA sequence	GAAAATTTCCAGTATCCACATGG
sgRNA2 sequence	ATATATTTATAAGAACCTCACGG

 

left_200bp	right_200bp
CCTTCGATGTGCATTTAATGTGTTTACAAAACTAGGACGTTGCTGTCCTGCGGGCACAGAAATCGACAAAAAAAAAAAAAAAAAGAAATCGTTGTCAAATGAGTGAATAAAATAAAACTATGTAGCTGGCTGTGAAATTTGTATATTAACTATTCACACAATTCAAGCGATTGGTAATAACTCGAAAATTTCCAGTATCC	GGTTCTTATAAATATATTTAAATATAGTTAATTAAATATGGCGCATAATACTGATGAGTGGATCTATAGGGTAAGAGGGCGTACAAAATGCAATGACTGCTTGATTCCTGGGCCGTCGCTTCGTCTTTCGAGTTACGAAGATAAATGAGATGCCATAAGTTAGACGACAACTTGCACAACTCCAGACAAAGACCAAGCGG

 

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