CR71438

CRIMIC CR71438

Gene CdGAPr

CG10538

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:19774874..19791882	38A3-38A3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTAAGACTTGAGTCTACCATTTACC	ACTTAGGACACCTTGCTCAAAGTTG

Images

Construct Design Sequences

sgRNA sequence	GCTCACCTTGTCCGACATGATGG
sgRNA2 sequence	ATATGCATTGTTCAGGATCGAGG

left_200bp	right_200bp
ATAGTAATGATGGGCATGGTAAATGGTAGACTTGTAGACTTGTGGGAAGAATTGCAGCCTTCGCATTGTTATTTATCGCGTGGGTTCTCGGCCACTGAAGGCCTAAAGAGTTAAGCACTGAGAATGCATTCACTAAATCTGAATCTAAATTCCCCGCTTCTTTCAGATGTCGCAACTGGCGTAGAGTGATCGATCCATCA	TCCTGAACAATGCATATTGTTATTGCATTTTGGGAGTTTCTTTAAATAGGTGTTAAGTTTCATTCGTTTAACCATACAAAAAAACGGAATTCAACCCTCTTGAATTCCATTTCGATTTCGATTTCGATTTGTTTTTGCCTGATTAGTGTCTCGAGTGTTTTTATATTTTTGTTTTTGCGACTAAACTTTGACGCATGTAT