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CRIMIC CR71439

Gene RhoGEF64C

CG32239

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:4692783..4796253	64B13-64B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGCTGACATTGATGATGAGC	TGCCATTTGTGAGTAATACACAGAG

Images

Construct Design Sequences

sgRNA sequence	TTAATTATGAGCTGCCCGTTCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGATAGTTTGCATTATGAAGATGTTCTGCCAGGACCCCAAATATTTGACAGCAGCGAGTCCTCTGCCAAATTATCACTGCCAGCGGATGGGTCGCCGCATGTCACTACGAGTCCTGTCAGCGTTTTACCCACTGGTGAGTACTCCGATTCTGGAATCCCGTCATGCCATAAAACAGTTGACTTTTAATTATGAGCTGCCC	GTTCGGGCTATTGCAAAGGCCAGCACTTTTTCAGCCAAGTTTCTTTCATTAGTTAACTCACTTGAATCACAGCAAAAAATAAGTGAAGTTCGTTAAATTTAAAAACTATAAGAAAAGTAATTAAATGCTGAAATCTACTTTCGAAATAAGCTGTAGCTTTCGTTAGAATCTAAAATTAGTTGAATATTTAAATGTAGATA

 

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