CR71440

CRIMIC CR71440

Gene Atg2

CG1241

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:2774338..2780667	62F3-62F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGGTAGCAACAGGTTGTTTGGAG	CTTTTAGCAGCACCATGAAGAAATACGTC

Images

Construct Design Sequences

sgRNA sequence	CGGGGGCGCAGAGCGGCTGTGGG
sgRNA2 sequence	TCACAGATCGACTAGGTTAACGG

left_200bp	right_200bp
TCATTTTATTTTCAAATGCCCCCTGCGAACGCTCCACCGCATTGCCCACACCAGGGGGTATTTTACGAAGTGCCCACACCTGGCCACACTGGTCGCAACTACAAAAAGAAGAAGAACAAGGGCCAACTTGATGGTCACTTGCTACTGCGCTTTATTAATTCATTATTTTCCAAAATAATGCACATATATACCCGCCCACA	TAACGGTAGCTATAGTTCACGGGGCTTACAGCATAACGCAATGTTTTCTATTGTTTTACTAATAATAAATAATGTACATAAAAGGAGCACTTGCACGAGACGGCGAGGTGACGAGGGACTTGGGATCCCGAACGGTGATTCCGACATGGGTGTGATTGAACTTATCCTTTTTAGCCATATTACAGTACATGAGAGTTATA