CR71442

CRIMIC CR71442

Gene CAH13

CG12309

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:10725270..	47B7-47B7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGCATCCTTCTGCCACT	ATCAAGTGGGTCCTTTCCCAAGTC

Images

Construct Design Sequences

sgRNA sequence	TTATGTGACTGAGTTCTTAAAGG
sgRNA2 sequence	GACCACAAATGGAGTGTCTATGG

left_200bp	right_200bp
ATTAGAAACGATTAGGATTAGTAGTCTTGCAATATCTTACTACTCCCTAAGACATTTGTTTGGGACTTATTTAAATTTTGTACACTTTATTATAACTAATCCACATGATCTATTTAACGTCTCATGATATCAAGATGAGATTAACAAAGTAGCAGGCTAGATTTGACTGTCACCTGGTATATAAGACAACAGAACCTTTA	ACACTCCATTTGTGGTCCATGCTGCACGAAGAACCGGTGGGGCTAGATGGTGCACATGAAACACCGGTCAAGGCCAACTGGTGGCCAACCGGAGTTCTCTCTGTGCCCGGAAGTGATTGAATCGCGTGCGTGTGGATTAAACAAGTTGCGTTTATATTTTCTAAAGCATAACCCCCCAATTCCCTTGACTTTTAATCCTT