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CRIMIC CR71443

Gene CAH4

CG10899

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	III:24856383..24857559	96B1-96B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGACCAGTTGCACTTTCAC	GTGGTGCACTCGCTCAGTCA

Images

Construct Design Sequences

sgRNA sequence	TCGATGTTCGGATCCTTCAATGG
sgRNA2 sequence	CATATTACATGATATTCACTTGG

 

left_200bp	right_200bp
CAAGGGATCGGAGCACTGCTTGGATGGCAACTATTACGATGGGGAGGTGCACATCGTTCACAAGAATGCCAGTTACAAAAGCAACAAGGAGGCCGGTCTGCAGCCGAATGGCTTTGCGGTTTTGGCCCTGTTCATAAGGAACCTTGAGGTAGGAAGTGCCTCTACGCTTTACCTATACTCTAGCCATCTTATTTCCATTG	GAATATCATGTAATATGTATTGTCCAAGTCACATAGTGTATTAAAATTATTTTAAGTGCATTTGTTGTTTTATGCAAAAGCTTAGTCCAAGTGCTTGAATGGAAGCTTTTTGCCGGCCGCCAGCTGATGGCGCATCCACCCTTTCCCATTCCCGTTCCCAACCCCAAAGATGCGAAATTGCATTTGCTATTTGCGTAGCC

 

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