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CRIMIC CR71447

Gene CAH2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:12177601..12183347	69A1-69A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGAGCTACAACGAGGACGAGATC	ATGTGCAATAGCGAGGACATCCAG

Images

Construct Design Sequences

sgRNA sequence	CAGAAATATATTCCATTAGGTGG
sgRNA2 sequence	TAAATCGTTAAATGCCTAGCTGG

 

left_200bp	right_200bp
ACGATTGGCAGTGAGGATTTGATCAACAATCGTGCCTATCCCGCCGAGTTGCACGTGGTATTGCGGAATCTAGAGTATCCGGACTTTGCCAGCGCCCTGGACAAGGATCACGGCATCGCCGTGATGGCCTTCTTCTTTCAGGTGAGTGCTGACTTTTGGAGTTTAAGAACATAGATCCTTATACAGAAATATATTCCATT	AGCTGGCGCTTAGGCGTCCAATTAAGCGCTGTAATTTACATTTTCCGCTCTTGTTCGCCGTCGCTGTTTGTTGTTCAAACACGAGTCCCGGTAATTTCCTTCGGCAAGGACTCAACTGCCGTCAGGACATCCATTCATATCCACACACACACACATATATATATATATATAAATATATATATATGGGCAAACAAACAGGC

 

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