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CRIMIC CR71448

Gene CAH3

CG11284

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:8681252..8688760	8A1-8A2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCAACAGCACGCGAGTCGCTAA	GATGTCTTAAGTAGTTGTGCCCG

Images

Construct Design Sequences

sgRNA sequence	AAGCGGGCTTAACCAATCCGTGG
sgRNA2 sequence	AGAATTGTATCCAAGTCATATGG

 

left_200bp	right_200bp
AGACTCTCGTTTCGCTCTGGATACAGGAAATTACCGCTTTTACACACTTGATATTGCTGGATATATCCGGTTATAGAAATTACACCCAAAACGGAAGATGCTCATGAATTCGCGTTTGTTTATATCCTTAAAGTAGCTGAACATTATTAACTCGATTTCTCTGCCTCTTGCAGGTGCATTTCCAAGCGGGCTTAACCAAT	GACTTGGATACAATTCTACAATTCCCGCTCCCGCTGACTTTCCTTTTTCCAACCTTTCGCGCAATTTTCTCCGCCCAATCGCCTATCTAAACTATAGCTATGTGTAAACATTTATCTGTCTATGGTTGTCTGTAAGCTGCTTTTGTTATTTGCCTCATGTACATAGCGTTATATACCTAACAAAACATACATATACATAC

 

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