CR71455

CRIMIC CR71455

Gene nAChRalpha2

CG6844

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:24485428..24491147	96A3-96A3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTGCGAAACCGTTCAGGCGAAT	GGTCTACACGGAGAGAAATCTAGC

Images

Construct Design Sequences

sgRNA sequence	CAGCAAATGTAGCGAAACGCGGG
sgRNA2 sequence	<br /> <b>Deprecated</b>: htmlspecialchars(): Passing null to parameter #1 ($string) of type string is deprecated in <b>C:\wamp64\www\pscreen\crispr\phpMyEdit.class1.php</b> on line <b>1168</b><br />

left_200bp	right_200bp
GATGCCAAGCGACTCTATGACGATCTGCTAAGCAACTACAATCGCCTCATCCGCCCTGTGAGCAATAATACGGACACGGTGTTGGTCAAATTGGGCCTACGGCTCTCCCAACTCATCGATTTGGTAAGCAAAATTAATTGGTATAATCATCGAAGTTTGGCGGGTGGGGGGATGTTTTTAATTAAGCTCAAGCGCCCGCG	TTTCGCTACATTTGCTGGCAAAACAATAAGAAGTGCGTAAAAGTTGCCAGCAACTTTCAGCAGCTCGTAAATAAAAAATGCAAAACAATAAACAGCAAGAAATGGGGCGAATCGCGAGCTGGCAAACAGAGAAATCCATGGGGCCATAATGACACACCCCCCCAAAAAAAAAGAGGGATGAGTGCTGTAAGTTAAAAGTA