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CRIMIC CR71462

Gene Pop2

CG5684

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:11998398..12002915	68F1-68F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCATGGACACCGAGTTTCC	GGAAACATATGTCAGCAAAGCG

Images

Construct Design Sequences

sgRNA sequence	AGGGAAACAAACGATAATATAGG
sgRNA2 sequence	TCTCATTTCAATTCGTATCGCGG

 

left_200bp	right_200bp
TCGGCGAGTTCCGCTCCACGGCGGACTATCATTACCAGCTGCTGCGCTGTAATGTCGATCTGCTGCGGATTATCCAATTGGGCCTTACCTTCATGGACGACGATGGAAAGACACCACCCGGCTACTCCACCTGGCAGTTCAACTTCAAGTTTAACCTGAGGTAAAAGTGTTTTGTTTATGCTCTAATGGGGGGTCCTATA	TACGAATTGAAATGAGAAACGCGCTGGATTCGAATGGAAAATTAAATGAATTGGAATTAATTTTCTTTAAATGTTTATTTGCATTCTTTGGTGTTTGGGCATGGCATATGCATATCATAGCTTGCTTGATTTCATATGACATCTTTTTAATTGTATTGATGTTCATTTTAATTTATGTTAAGTTCACGCTCTTCATTTAT

 

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