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CRIMIC CR71464

Gene Rcd-1r

CG9573

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:9012885..9014068	29F7-29F7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGAGAGCTAAGCTATGCTATGAC	TTGGAGCCTGCAGCTTAGCACTTA

Images

Construct Design Sequences

sgRNA sequence	CGACGGACGATTGAGAAACGTGG
sgRNA2 sequence	ACTGCTCGACCGAATAAACATGG

 

left_200bp	right_200bp
GACGAATTCCAAGCTGCCAATCTAATCAATGGTGATAAGAAACAAAATTCTAGGAGACATTCGAACAAGAAATTATTTATTAATTTATGGCATTCGTGCGTTCCTCGGCTCTGGCCACATTGTTCGGCCACGTGCTTTCGGTCCTTTCTCGCTTGAAGCTTATATCGATAACCACAATAGACGCCAACTCGAAACCACGT	ACATGGCCTACATAATCTACAAATGAAATTATGATATTACTAAAATTGACGGTACAATAAAAAATATCAAATATAAAATATCATAGCTCCTAGCTCATATTTATTAATAGTCACACTAGGCATACATCTTGACCAGCAAGAGACCCAGAACGCTAGACGCGATGGCAATAGTGCTGCTGCTCATCTTTCCGGCCGGATTC

 

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