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CRIMIC CR71465

Gene Pos

CG2053

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:32038330..32039753	100E3-100F

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCAGCCGAAATGATATGGCG	GTAACTGTAATGTGTGTGTGCTACTGAG

Images

Construct Design Sequences

sgRNA sequence	GCACTCATTTAAGTTCTGCTTGG
sgRNA2 sequence	AATTAGGGGAAGGAAAACTAAGG

 

left_200bp	right_200bp
ACGTTATTCGGTTGAAGAATAGTTTTATTTTTACGTAAACCAAACCAGAATTGCTTAGATAAATGAGTCAAAGTATAAAAGCATGTAGTACTAAACATTTCTCATAAAAAATATCACTTATACAAAAACTACAATCGATCAGCTTGACTTAATACATATTCCACATCCACGGCGAAAATATTAATTACTCCTCACCAAGC	TTTTCCTTCCCCTAATTGTCCTAATCAATGCATACAATTGGTGAACCAATCAGATGTTTTATTTGGTTGCTTCTGGATATCTTTGGTTGGAGGATATGTAAGGGTTCTCTTGAAGCATTAAGTTAGAACAATGCAATAAACAGATCGTAAATATTTTGATACCATTTTTCCACCGTAATTGACCCGACGATACTTTCAAT

 

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