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CR71469

CRIMIC CR71469

Gene Cul6

CG11261

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:..			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
		TGGATTGAGAACGACGAATGCG	TGATGGCTCAGAACGGATATGCGT

Images

Construct Design Sequences

sgRNA sequence	TTACTGATACATTCTATTAATGG
sgRNA2 sequence	TTTATGAGGAGCTTAAAGATCGG

left_200bp	right_200bp
GTTACAAATTCTAAATCTTAACCTTAAAAAACACAAAGCTCCCACACATTTTAATTATTACTTCGGTTCTGTGATATATTATTATTATGTTTAGTTAGTGGAGCTAGTATGTTTTTGCGTAGAGCTATCGATAAGAATCGTTGAATGGTTAATGTCAGGCGCTGGAAATTATAACATCCCTAAACCGATAAGACCCATTA	GATCGGGTAAAGCCACAAGTGAGTTTTATCAAGAAAAGACTTGACTACCTGGTGGAGCGTGAATATTTGGAACGTGATAATTATTATAATATTTACAGATATTTGTAGTATAGAAAATGAAGGTATACGATTTTAATAATTTTAAAAATATTTTGTATAATTAACAAAACGAAGCAAATAAACAATAACAATATATATAT