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CRIMIC CR71469

Gene Cul6

CG11261

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:13009399..13011685	69F5-69F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGATTGAGAACGACGAATGCG	TGATGGCTCAGAACGGATATGCGT

Images

Construct Design Sequences

sgRNA sequence	TTACTGATACATTCTATTAATGG
sgRNA2 sequence	TTTATGAGGAGCTTAAAGATCGG

 

left_200bp	right_200bp
GTTACAAATTCTAAATCTTAACCTTAAAAAACACAAAGCTCCCACACATTTTAATTATTACTTCGGTTCTGTGATATATTATTATTATGTTTAGTTAGTGGAGCTAGTATGTTTTTGCGTAGAGCTATCGATAAGAATCGTTGAATGGTTAATGTCAGGCGCTGGAAATTATAACATCCCTAAACCGATAAGACCCATTA	GATCGGGTAAAGCCACAAGTGAGTTTTATCAAGAAAAGACTTGACTACCTGGTGGAGCGTGAATATTTGGAACGTGATAATTATTATAATATTTACAGATATTTGTAGTATAGAAAATGAAGGTATACGATTTTAATAATTTTAAAAATATTTTGTATAATTAACAAAACGAAGCAAATAAACAATAACAATATATATAT

 

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