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CRIMIC CR71471

Gene CG10333

CG10333

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	II:18606832..18609550	36F7-36F7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	ATGTCCTCTTGGTCCTGAAGTACG	CGCTAACCTAACATCCGCC

Images

Construct Design Sequences

sgRNA sequence	TTCGCTGACCTATATACGAAAGG
sgRNA2 sequence	CATTCCATGCTGGGAGCGAGCGG

 

left_200bp	right_200bp
TACATACTCTTGTTCCTCCAATTACAGCTTGATTCTGGGGACCAGTGCGGACAGCGAAAAGGAGGAGGACATAGTCGAGTGGCTGAGGGAGGTGGGCATGCCGGCGGACTACGTGAATCGGCTGGCGCGCATGTTCCAAGACATAAAGGTAGGTTTTACCAGACTGATGTTAGGAGCAGGTTTATTCATTATCCCCTTTC	GCTCCCAGCATGGAATGCCTAATCACACACAAACACTCACACGGGATCGCAAAACTATAACTAAAATAGCAATACTTTATGTTTAAGTTAATGAATAAATTTGGTTCTACACATAATGAAAGCGAATCTATTGCAGTTTTATTTTTAACTGGTTACTGCAACTGAAGGGCGAATAAAAATCGAATGCATTTCATTATACC

 

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