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CRIMIC CR71477

Gene Dus2

CG1434

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:14237988..14240152	12E5-12E5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAAAACACACCAAGCGAAATTGC	CTGCGTGAACCCGACATTAGGAAA

Images

Construct Design Sequences

sgRNA sequence	TGTTGCACGCCGTTCAATTTGGG
sgRNA2 sequence	AAAAAAAAACAACTAAATAATGG

 

left_200bp	right_200bp
ACAATATTCAGTAGGCGGAATACAATCAAACAAAAATATGCAAAAATATTCACTAGCACAAATAAATCATATCTGCATAGAATATGCAGTTCTTCAATAATTATTCAATCATATTTATATGGCGTAGAAATAGTACCTTGCATGACGGCTCCACGTGTCGTGCAGTGTCCGCCATGCGCTGACGTGCAGAGGGCCCCAAA	TTTAGTTGTTTTTTTTTTTTTTGATTATTGCAATGGCTGTTGCAACTGCCTTAGGTGCTGGGAGCAGTATCTGGACTACCTCCATTGGGTTAACAAAGTTTGCATCTGAATAAACAAAACTGCAAGTGTGCAGTGCTTTAAATATATGTATGTATTTAGATAAATAAATTATATGAGCTGTTTTAAGTTTATAAGGAGTA

 

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