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CRIMIC CR71478

Gene Dus3

CG10463

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:19758879..19760986	38A2-38A2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGACTGATTCACTGTGACCACGGA	AGTTGTTTAGGTACCAGCGAAGAG

Images

Construct Design Sequences

sgRNA sequence	CAATAAACAATCGCACGTGTTGG
sgRNA2 sequence	GAAGGCATTCGCCTTGTGCTTGG

 

left_200bp	right_200bp
AATTACCAATTTAAGGGTGGAGGAGAACTCATCATTGCCACGTAAGTCGTGGATTAATTTTAGTATTTATTAGTTAATAACCAGTGAAATCCATTCAATTCACAGTTATGCTGAACGCGACCAATATCTTTTCTTCTATCGATAAATCATCACAAGCAGCTGATTGACAAAAACAAGGTAGAAGAAGACGCATACCAACA	ACAAGGCGAATGCCTTCTAGGTTTCTTTCGTGTGTAAATTTGTATTTTTGAATTTATGCGTAGTCTGACATAATTAAATTTATAATTTAAATGAAATGAACAGCTTGTTTAAGATATAATAATATAACATATATATATAGATATATATATGTTATATAAGTATATATTACATATGTATATATGTTAAGCCATTCTTTTAA

 

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