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CRIMIC CR71481

Gene CG4849

CG4849

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:27963415..27966958	98B6-98B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCACACTTGTCCTGCAGGATG	GAGCAGGAAGGCTACGAGT

Images

Construct Design Sequences

sgRNA sequence	GCGATCAATCTGCACGTCAGAGG
sgRNA2 sequence	GATTTGTGGGAAGTCTAAAGCGG

 

left_200bp	right_200bp
AACTTCTCGGACGAGGCCACCGCCGCCATGCGAATGAGCGATGGTGTCGTGCTGTTCATCGATGCCGCCGAGGGCGTCATGCTCAACACGGAGCGACTGCTCAAGCACGCGGTGCAAGAGCGCCAGGCTATCACCGTTTGCATAAACAAGGTGAGGGTTTGGCCTTAAGGTGACACTAAACTTTCTAATGGTTTCCTCTG	TAGACTTCCCACAAATCAGGAATCGGGAAAACAAATAAAACATTTAGACATACTTTCCGAATTTCAATTGCGAACTTTAAAGGGAGTAGTGTTTTTTTTTACTATATTTACTAAATATTTATTTGAAGCCTTTTCGGGACAAATAATTTACAAAATAGTATACAGAAAACAGCTACAGTTGTGATAGTTTCTAGGACTTT

 

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