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CRIMIC CR71487

Gene fd19B

CG9571

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:20091428..20092408	19B1-19B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTAATCCGAAAACGTTTGCGAC	AACTAGCATATGGACTTTGCTGG

Images

Construct Design Sequences

sgRNA sequence	ACATTGCAATATTATTACGATGG
sgRNA2 sequence	TTCCTTCTTGGCGTAGAACGCGG

 

left_200bp	right_200bp
ATCAATGAATGAATCCGAAGGCTTTCATGAATCTGTTTGCGCGGCGATTAAAAGGGGAATGGATCGCTAATCCGCGTCCCAGGATACACGGGTAATCCTCGAGTTCAGCCTCTTCCTCTCGTATAAATAGATCGGCGGCCGGTGGACTGGTCACAGTCGTTTTACGAACTCAAACAGTGACACACATTGCAATATTATTA	ACGCGGCAATGTTTTTAGCTTTAAATCATGTAGGGTAATATCCATAGAAACTATGCCCAGGCTTTAAAATATATCGTAGTAAACGTTACGTGCATTCTCATACAAAACTTTAGGTTGTTTTCAAATAAAGATGATTCGACATTCGAAGAATAGTGCGAAATTTTTATTGTATTTTGGTTTAGTGATTTATATATTTTGCT

 

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