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CRIMIC CR71488

Gene FoxL1

CG1132

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:4018878..4021279	64A4-64A4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGACCCTCAGTGGAATCTACAAG	CCCATATCTATATCCTCCGCTGGT

Images

Construct Design Sequences

sgRNA sequence	GGGATTAAGTCGGTGAAATATGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTTGTTTTTACAATCCTACAAAAATAAATGTTCCGCCTTGTCTTAAATCGAGGAAAATTTAGATGACATTCTTGGCTAAATCAAATGCCAAAATAAACAACCGCCACATTCCAAGTATCTGTACAAACCAAAGAAGTTTTTATCCCGAGAAGAAGTTTTTATCACGAAACTTTTGCCACAACTGGGATTAAGTCGGTGAA	ATATGGAGCATTAATCCAATTTCTTAATGTGAAAATATATAAATATCACGCAGATTACTTGGTAATTATCCATATGAAATGGTTTCATTTATTGGCTTAAATTTATTAGTCATATCTTTAACAAATATTAAAATATATATGTATATATTTCCTTTCCTGAATGAACAGAGTGCATAAAAAATGGCGGCCAGATATATAAA

 

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