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CRIMIC CR71491

Gene fd59A

CG3668

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:22868159..22870706	59B4-59B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACGTCGGCAGTAGAATCACAGTT	CTATAGACGTGCGATGCTTCAGAT

Images

Construct Design Sequences

sgRNA sequence	TTCGCTGTTACCGTCGTTTCCGG
sgRNA2 sequence	GCCAATTGGTTCAAGCGAGTGGG

 

left_200bp	right_200bp
TTTTTCATAAATAAATGTTTGAGTTATGTAGAAAAACATTTTGGGCGAGAAGATTTTCGTTTTCGTTGCGATTCAGTTAACATTTGGCCAGCGGATTCCGCCTTCAACGGACGCGACGACGGACTAGGCGCTAGCCAACTTTCCACTTTCCACACCACTGCTGGCCATATGTACAGTTTATCCTAGCAGCTAAACCGGAA	CGCTTGAACCAATTGGCTTGTGTACAGTCCATTGAACGGTGATTTCAGTTTCGATCTAAGCACGTTTTAACACTCCACTAACACCACCACCAGCAATCCCCAGGAATCAAATCCGAGCAGCAAGTAGTGATCAGTAGGTGTATCCGTAGGATGTAGGAGCCAGCACACGAGTTTTTAAATGCGACCAAATCGAAGTCAAC

 

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