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CRIMIC CR71504

Gene Ir7e

CG42293

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:7879800..7881679	7C1-7C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGAGGAACTAGAAGCTCTGT	GCCACAGTCACCCACACTTG

Images

Construct Design Sequences

sgRNA sequence	GCGCTGATGTTCATCCCGCCGGG
sgRNA2 sequence	AAAAAGTTTCCTTAAATAGATGG

 

left_200bp	right_200bp
TTGCATTCTTTTCACATGATTAGTTTTTTTTTTTTGTCAGTGGTTTTGACCCAATTTTTGTGTGTAAGTGAAACAACCATTAATCATGAAACTTTTCCGGGCAATTAGCTTGAAAGTTACACAAAACTTCTAATATGATTCGGAGGCAATATTCTGGTCAGGATATGGATATCCTTCATTGAGCCACCTCATGTCCCGGCGGG	ATTTAAGGAAACTTTTTACGTAGTTTTGTACTTAAAGATAAATTTAGCTAGCTATATTTCGTAGAAGTGAATAAGTAGCTTTATCTATATCAGAATTCAAGTTATTTTATTACAAGCGAAGTTATAAAACGATTTACTTTCAATTATTTATTTTTTTTTAATGTAATTAAAATATTGTATAATTTAATTATTTGCGAATT

 

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