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CRIMIC CR71508

Gene Ir75a

CG14585

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:17815570..17817921	75A2-75A2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGATGTAAGTTATGTGAAGGAGCAG	TTCAATTCGAATGTGGCCGTGCAC

Images

Construct Design Sequences

sgRNA sequence	AAGTTCTAGCTATTTCTTAGTGG
sgRNA2 sequence	ACTATATTCTATTTGCACCCAGG

 

left_200bp	right_200bp
ATGGAAAAATTATTGGGGGTCAATTGAATGTAACTGGATCCCACGAAATGTCTTGTGATCCATTCGTATGTCGCAGGACTCGACATTTAAGTTCCTTGCAGAAGCGATCTAAATACGGAAATAGAGAACAACTTACGGATGTTGTCCTGAGAGGTAAGACATTATTGCATACATAAAACATTTAAGTTCTAGCTATTTCT	CCCAGGAGAAAATAAAAACACTTTGCGTTAACTTTTCGCATCGCAAAATTATGCAGGGCGACGCTTTCCTCACTTCCTGTTTTCGCGCCGGAAAATTGCAGCACGGCTCGTTAAACGGAGGGATGAATAAAGGGGTTTGCAAAGGACGTGGTGGCCATGTTTCCATGCGATTTTCGTATTTGCATTAAAAACTTTTCGTG

 

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