CR71511

CRIMIC CR71511

Gene Task7

CG9361

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:910329..9412293	85D15-85D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TTCGATTCCCTGGAGTCACCAAC	AAGGCTACCGACCAGATGATACGA

Images

Construct Design Sequences

sgRNA sequence	ATTCTGTTGTAACTTTCTTTAGG
sgRNA2 sequence	TGGCTCTGGCTCCTACAATGAGG

left_200bp	right_200bp
GTTAGAAAGTACAATGTGACTGACGAGGATTTCCGTGTGATGGAAATCGTCATCATTGAAAATAAGCCCCACAAGGCCGGACCTCAGTGGAAATTCGCTGGAGCTTTCTATTTCAGCACGGTTGTACTGGCAATGATAGGTAAATTAATTATCTATTAAATATGATTTATTGAATAGATTATAATTCTGTTGTAACTTTC	TGTAGGAGCCAGAGCCAATGGATCACCAAATCGTAGTTACAATCCTGTAGAGAACCATCCGCCGCCAAAATTTGGTTGTTAGACAAACCTTCCTCCCTACGTAGATTTTTAAACCAGGATGGGTCATAATACATATAAGTTTGGAGAGCAAGGTTAATAGTCTTTAAAAGGCAGTTTTTGCTTAAGAAATAATCGACCCA