CR71518

CRIMIC CR71518

Gene tko

CG7925

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:2442313..2443976	3A3-3A3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCCAAGAGAGAAGCATCCTAAACTCG	GAACTTCGTAGCTGTGAAGAGTGG

Images

Construct Design Sequences

sgRNA sequence	AGCGCCACCAATCCGAGCGACGG
sgRNA2 sequence	TGAACAACGTGATTAGGAAGTGG

left_200bp	right_200bp
ATGCACCCACCATGTTTTAGCGAGTGCACCTACCATACGTGACTGCCATGCGTGACTATCGACACCTGTGCCAGCTCTAGGACAGCAACAGCTGATTGTCACATTAGAATTGAATTGGAATTAGATTATTAAATATTTTAGCGCTTTTATAACACAAAAGGCCGACTAAATAGCTTCCGAGCAAGCGCCACCAATCCGAG	CCTAATCACGTTGTTCAAATGCATTGCTTCACAATCAACAGACGGATGCGTCGTTCTTATTATTGTTTGTTTTTTATGGAAAATTATGCAGCTCAGCCGGCTTAAGAGGTAAGTGAGTGAGTAGTGACAAGTGAACGGGCTCAGTCAGGATCTCCTTTCGGTTGGCTGCATTCTCGTAATCTTAAGTCAATAGGCTGTCG