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CRIMIC CR71543

Gene CG31624

CG31624

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:21199045..21200375	39B1-39B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCATGGCTAGATCGACTCGG	ATGTGCATGTTCCAAGGCTGGGTA

Images

Construct Design Sequences

sgRNA sequence	GAACAGTACAATTACGAACTAGG
sgRNA2 sequence	CCGATGCTTATGAAGCCTAGAGG

 

left_200bp	right_200bp
AAGAATATATATACTTTGTATGTTCGGAAACGCTTCCTTCTGCCTGTTACATACTTTTTAACGAATCTAGTATACCCTTATTATACCCGTTATTGCTTGTTGTTGTGCTCCCATTTTGTGTGAAACATCTAATAGAAAGTAAAGTAACAACGGTGGCCCTGTGACAACTGTTTTGATATATGAGAACAGTACAATTACGA	TAGAGGCATGAAAGAAATCAGACCCACACATTTGACCACACTTAACATTTCGCACACGCACACACTAAATAAAAATAAATATGTAAAGTCTCTGTATTGGGGTGGCCAGGTAAACCCTTTTATCGTTAAGATTTGCCTAAGACCAACAACACCCACAACAACATACTGATTACTGCGAGATAAACTAGAACGCAAGCCCT

 

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