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CRIMIC CR71549

Gene SPH93

CG6639

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:17454263..17455923	36C9-36C9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCCTGGGAATGATCGTTACCAGT	CTGGGAAGGAGATTGCATATGC

Images

Construct Design Sequences

sgRNA sequence	CAGAAAACTGAGTGAACGAACGG
sgRNA2 sequence	TGCCCTTCGATTACAGGAGTGGG

 

left_200bp	right_200bp
TTCCAAAGAATTCGGAAATATCTCTTTCGCAATCTCTTAACTTAAGCGTTTTCTGTGCAATCTTTGGGAATAACACAATTTATGTGCATATGCATGTACATATGTACATGTACATATGTACTTCGAATACATCTTTGTCGGCTGATAAGCTTTTAAGCTGCTCAGCAAAGAAATATAATGATACCCACTGCCAGCCGTTC	AGTGGGTGATATGTCAAAAGGAACGACATGATGCAATAAATGATAAAAGCGCTTAAAAGTGTACATTAAAGTGCACAGTAATGCAAACAAATTGTTATTGTAATAGTGCTGTGTTATTCGTTTTATAGATGGGAAAGTATAACGATCATGAGCATGAGCATAAATAATCTTACTCATTAATGTCAAACAGTTGCTCCTCC

 

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