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CRIMIC CR71554

Gene edin

CG32185

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:17494880..17495300	74D1-74D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTTAGAATGTGTTCAGGTGTTCG	GAAGAGTATGTTCCACTTCAGTCTG

Images

Construct Design Sequences

sgRNA sequence	TTGTTGGAGAACATCTTAGCTGG
sgRNA2 sequence	CGGGAACATAAGTATTAAGTTGG

 

left_200bp	right_200bp
CTATTGTTGTCATATGGCGCTGATAAGGATCCCCGACCGGGATGAACCTTTAAAAAATCCCCGTTGGGTTGATAAGAAACAATGGGATGACAAGTAGTAGTCCCCGTTGAGGTGAATCCCTTTAGGAAGCTCATTGCTCCTATAAAACCGCAGACCATTTCTATGCTTCTCATCACTCTGATTTAAACAATTAGCCAGCT	AGTTGGTTACAACTTGATTCATGTGAATAAATACGTGTGTTTACCCAAGCGTGCTACTGTTGTTTTCCCATTAGTGACAAATCCTCCTCGGCGGATCCCATCGAAGCCCAAAGATCTTTGCGCCTGACCAAAACAAACAGTAGCCGATGCATCGGCTATTTACCCAAAGTAACTCAACTGAAAAAAACAACAACAGGGGG

 

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