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CRIMIC CR71558

Gene IntS6

CG3125

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:5738663..5747254	5B4-5B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACCAAATTGTTTGGCTCGAAACTC	CTATTGCTAACTAACGGCTAGCTTAAG

Images

Construct Design Sequences

sgRNA sequence	ATGATTGATCCTGCGTTCGAGGG
sgRNA2 sequence	GGATGACCCCGCGGGCTCAGTGG

 

left_200bp	right_200bp
TTTAATTTCATTTTGTGTGAAGATTTGTGATATAGAAGAGCCAAGAAACCATAACGCCAAGCCGACATTCCTTTACTCTACGATATAACAAAAAGTATAATCGGAATTAGTATTTTAATTTGAAACATTTGTCCGTTTCTTCTAGGATATCAGAGAGAGACGGAAAGACACGCAAATTGGGAAGAGGAAGAGGTCCCTCG	AGCCCGCGGGGTCATCCAATCCATCTGTCTGTGATGTCAACAAAATCGAAATCGAAATCTAAACCGAAACTGGGAAGGGGAAGAGCAGCCGCGGAAGCCAAGAATTCTCGTCAATTTCCATATACTGTAAATAATTGCCAGGACAGGTAGCCCACAGGATCTGGATGGGGATCAACGCATACCAAACAAGCAACAATAAT

 

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