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CRIMIC CR71559

Gene defl

CG18176

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:9723423..9726706	67C5-67C5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAGCAGTGTGACCTTAGCAGATT	CGCGATGATGCCTCATTTGTCCTA

Images

Construct Design Sequences

sgRNA sequence	GTCAGGTGAGACATGGTTACAGG
sgRNA2 sequence	TTTAGGCATTACATCCAAAGTGG

 

left_200bp	right_200bp
TTGACAATATAGACTTTGGATTCAAACGTTTTTTTTACTGTTAAGTGACCAGCTATCGCTGCAATGATACCGGAGTTTCCTACGAACGACAAATTAGCAAGAAGAAGCATTTGCAGCCGGTTTGTTTTTGTGTCGTTTGGCTAATGAAAACCTCAATAAACACGATTTATCTGCGATAAATGTGAGTGAGGAAACCTGTA	AAGTGGAAATATACGTTTTTCATCAAGAAATTGTGTTTTCTTAAATGTCTATTTATGTAAAATATTGATAAGCGTTAAGAAAATGTGCACCAAAAATCAGATAATAATATTTCAAAACAAAAATAAATATTAATTTCCAATCGGAGTGTTAAATGCTCTTTGAATTAGACAATGTATCGATACTATCGAAACAGGTGGGT

 

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