CR71560

CRIMIC CR71560

Gene IntS8

CG5859

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:16850437..16854104	53D13-53D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTTGTGTCCCAAACGAATAAGGAGAAC	AAGTAGTTCATTCGTGTGTTATCTGG

Images

Construct Design Sequences

sgRNA sequence	ACAATCGATGTATCTATCGATGG
sgRNA2 sequence	AACGAGAATCTGCCATGGTGCGG

left_200bp	right_200bp
GCTGAAGGTAGCCACTTGGCTGAAGTGGGATCTGGATGCATTGGAGAAGAACCTGCCTGTGATTATGCAGCTGGCCTTGCTGCGCGATCTGTGCACCATAAGCTACGGCTGCTCCTTAAGTATTCCCCTGCCCAATGATTTTGACGCGAGAATTTGTATGTTAATTCCTAGCCTCCACATATACTTCTCTATATCCTCTG	GTGCGGAAATCGAGATTTCTGCGTGCCCTGGCCAAGCAGTATTTGCTGTAGTCGCAGCCCCCACAGTCCAAACTCTAATTCAAAATCCAATATCCATAATCCAACATCCCAACCTCACCCGCACCCACAAGCAGACCAACTAGCATTAAGCTCAAAGATGTAGTTTATATTGTATGCATAGGCTTTACGGTTAAGTTAAG