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CRIMIC CR71561

Gene IntS10

CG1120

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:3806710..3809090	63F1-63F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGCAGATGAGGAGAGAGTCGCTT	TGCATGTTTAGTACACAACTGGTC

Images

Construct Design Sequences

sgRNA sequence	TTGGCTCGGCATGTTTCTTCGGG
sgRNA2 sequence	TGTGATTAAGTGAAATAACTAGG

 

left_200bp	right_200bp
TGTAACTAAAACAAGCGCACTATGCTAAACAAGCGTTTTGAAAATAATTGAAATCTCTAGCTAGTTTCTAGATCCAATAGTTCCCATCCCCAATAGAAAAATATGGCCAGTGCTAGTAAATGAATTCCTAATAGCCGGCTAAATGGTTTGTTTTTGTTGAAACTAGGAGAAAATTAATAAAAACAAAAGATATACCCGAA	ACTAGGAGGTAACATTTATAAAATATCTATTTTCCAACTATCTTACACATTGTGTGTTTGTTTAATAGATACGACTATTATATTTAAGTTCACCAGTCTAGACTGGTGTATTTCGCTTATCGAATAAACAAATATTTATTTTATCAGCTGATAACATAGTTAGAGGTTGCATTTGATATGTTTCGATTTAGTCATACGCA

 

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